Thiosulphate oxidation and cytochromes in Thiobacillus X. 1. Fractionation of bacterial extracts and properties of cytochromes.
نویسنده
چکیده
In a preliminary communication (Trudinger, 1958) the separation of some soluble cytochromes of the autotrophic sulphur bacterium Thiobacillue X was briefly reported. Addition of thiosulphate to intact cells results in the rapid reduction of the cytochromes and the present work was undertaken to attempt to characterize the cytochromes and to obtain some information on the electron-transport chain involved in the oxidation of thio-
منابع مشابه
Thiosulphate Oxidation in Thiobacillus
bacterium Thiobacillus X. A fifth fraction (acidic fraction) contained two additional cytochrome components with x bands at 550 and 557 msi respectively in the reduced state. 2. All the cytochromes appear to be ofthe c type, are labile to heat and acid and do not combine with carbon monoxide. They are auto-oxidizable to varying degrees. 3. Cytochromes 550 (1) and 557 (1) are basic, and are adso...
متن کاملThe Extractability of Inner-Membrane Proteins from Salmonella typhimurium Intact Cells, Spheroplasts and Inner-Membrane Fragments by Non-Denaturing Detergents
The effect of Triton X-100, Na cholate and Tween 80 on the solubilization of integral membrane proteins in intact cells, spheroplasts and inner-membrane fragments of Salmonella typhimurium was studied. The detergents were used in various concentrations (1.6 to 64 mM) and cytochromes b and d were used as marker to monitor the solubilization of membrane-bound proteins. Results showed that no inne...
متن کاملThiosulfate Oxidation and Electron Transport in Thiobacillus novellus.
Aleem, M. I. H. (Research Institute for Advanced Studies, Baltimore, Md.). Thiosulfate oxidation and electron transport in Thiobacillus novellus. J. Bacteriol. 90:95-101. 1965.-A cell-free soluble enzyme system capable of oxidizing thiosulfate was obtained from Thiobacillus novellus adapted to grow autotrophically. The enzyme systems of autotrophically grown cells brought about the transfer of ...
متن کاملMechanism of oxidation of inorganic sulfur compounds by thiosulfate-grown Thiobacillus thiooxidans.
Thiobacillus thiooxidans was grown at pH 5 on thiosulfate as an energy source, and the mechanism of oxidation of inorganic sulfur compounds was studied by the effect of inhibitors, stoichiometries of oxygen consumption and sulfur, sulfite, or tetrathionate accumulation, and cytochrome reduction by substrates. Both intact cells and cell-free extracts were used in the study. The results are consi...
متن کاملReduced nicotinamide adenine dinucleotide oxidation by Thiobacillus neapolitanus and Thiobacillus strain C.
A. J. Smith, J. London, and R. Y. Stanier (J. Bacteriol. 94:972, 1967) reported that a number of obligate autotrophs, including Thiobacillus thiooxidans and T. thioparus, lacked reduced nicotinamide adenine dinucleotide (NADH2) oxidase activity, although this activity was readily detected in the facultative autotrophs T. intermedius and Hydrogenomonas eutropha. They suggested that the absence o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Biochemical journal
دوره 78 شماره
صفحات -
تاریخ انتشار 1961